cd11b m1 70 anti mouse ly6g anti mouse ly6g 1a8 anti mouse ly6c anti mouse ly6g 1a8 anti mouse ly6c hk1 4 anti mouse f4 80  (Bio-Rad)

Journal: Frontiers in Oncology

Article Title: The development of pancreatic cancer is accompanied by significant changes in the immune response in genetically predisposed mice

doi: 10.3389/fonc.2025.1603293

Figure Lengend Snippet: Immune cells infiltration of pancreatic tissue with PC (Kras/Cre mice) and control (Cre mice) after Sal or CER injections, (A) representative images of CD20 + , CD3 + , CD4 + , CD8 + cells staining; scale bars represent 50 µm, (B) median number of CD20 + , CD3 + , CD4 + , and CD8 + cells, (C) representative images of staining CD11b + cells (scale bars represent 50 µm) and percentage of mice with CD11b + cells staining, scale bars represent 50 µm, n = 5 mice/group. Kras/Cre, mice with Kras mutation; Cre, mice without Kras mutation; Sal, saline; CER, cerulein; hpfs, high power fields. Statistically significant differences (p value < 0.05) between mice are marked * (for details see the text).

Article Snippet: In the next stage, the slides were incubated with rabbit anti-mouse antibodies CD11b (Biorbyt, Catalog Number: orb31554, dilution 1:100), CD20 (Biorbyt, Catalog Number: orb374711, dilution 1:200), CD3 (Biorbyt, Catalog Number: orb348965, dilution 1:100), CD4 (Biorbyt, Catalog Number: orb4830, dilution 1:100) and CD8 (Biorbyt, Catalog Number: orb323288, dilution 1:50) for 30 minutes at room temperature.

Techniques: Control, Staining, Mutagenesis, Saline

Journal: Frontiers in Oncology

Article Title: The development of pancreatic cancer is accompanied by significant changes in the immune response in genetically predisposed mice

doi: 10.3389/fonc.2025.1603293

Figure Lengend Snippet: Immune cells infiltration of pancreatic tissue with PC (Kras/Cre mice) and control (Cre ctr mice) after FMT and sham treatments, (A) representative images of staining CD20 + , CD3 + , CD4 + , CD8 + cells, scale bars represent 50 µm, (B) median number of CD20 + , CD3 + , CD4 + , CD8 + cells; (C) representative images of staining CD11b + cells (scale bars represent 50 µm) and percentage of mice with CD11b + cells staining estimeted as a weak (≤ 5 positive cells) or strong (>5 positive cells in site of view).; Kras/Cre, mice with Kras mutation; Cre, mice without Kras mutation; FMT, fecal microbiota transplantation; n = 5 mice/group; Statistically significant differences (p value < 0.05) between mice are marked * (for details see the text).

Article Snippet: In the next stage, the slides were incubated with rabbit anti-mouse antibodies CD11b (Biorbyt, Catalog Number: orb31554, dilution 1:100), CD20 (Biorbyt, Catalog Number: orb374711, dilution 1:200), CD3 (Biorbyt, Catalog Number: orb348965, dilution 1:100), CD4 (Biorbyt, Catalog Number: orb4830, dilution 1:100) and CD8 (Biorbyt, Catalog Number: orb323288, dilution 1:50) for 30 minutes at room temperature.

Techniques: Control, Staining, Mutagenesis, Transplantation Assay

Journal: JCI Insight

Article Title: β -Catenin disruption decreases macrophage exosomal α -SNAP and impedes Treg differentiation in acute liver injury

doi: 10.1172/jci.insight.182515

Figure Lengend Snippet: For APAP overdose–induced acute liver injury, male mice were i.p. injected with APAP (300 mg/kg BW) or the same volume of PBS after overnight fasting, and the mice were sacrificed 24 hours after injection. For D-GalN/LPS–induced acute liver injury, male mice were i.p. injected with 600 mg/kg BW of D-GalN and 30 μg/kg BW of LPS, or the same volume of vehicle, and the mice were sacrificed 5 hours after injection. ( A ) qRT-PCR–assisted detection of β-catenin in the liver tissues ( n = 4–6 samples/group). qRT, quantitative reverse transcription. ( B ) Western blot analysis of total β-catenin/p–β-catenin (Ser552) expression in the liver tissues. ( C ) Immunofluorescence images of staining with antibodies against CD68 (red) and β-catenin (green). Nuclei were labeled with DAPI (blue). Scale bar, 100 μm. ( D and E ) The expression of β-catenin in hepatic macrophages was examined by flow cytometry ( n = 7–9). ( F ) Immunofluorescence images of staining with antibodies against CD11b (red) and active β-catenin (non-phospho-Ser33/37/Thr41) (green). Nuclei were labeled with DAPI (blue). Scale bar, 100 μm. ( G ) The proportion of hepatic CD25 + Foxp3 + Tregs gated on CD3 + CD4 + T cells was analyzed by flow cytometry ( n = 10 samples/group). Scale bar, 100 μm. Data are presented as individual values and represent the mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001 versus nondiabetic controls by 1-way ANOVA in A , B , and G and 2-tailed Student’s t test in D and E .

Article Snippet: CD11b and active β-catenin double-positive macrophages were identified respectively by primary rabbit anti-mouse CD11b (AF6396, Beyotime) and rabbit monoclonal Ab active β-catenin (non-phospho-Ser33/37/Thr41) (8814, Cell Signaling Technology).

Techniques: Injection, Quantitative RT-PCR, Reverse Transcription, Western Blot, Expressing, Immunofluorescence, Staining, Labeling, Flow Cytometry

Journal: JCI Insight

Article Title: β -Catenin disruption decreases macrophage exosomal α -SNAP and impedes Treg differentiation in acute liver injury

doi: 10.1172/jci.insight.182515

Figure Lengend Snippet: ( A ) Representative pictures of H&E staining of liver sections. ( B ) Hepatocellular function, as assessed by serum ALT/AST levels (IU/L) ( n ≥ 5 samples/group). ( C ) Representative images of TUNEL-staining liver sections and quantification of TUNEL-positive nuclei per high-power field for at least 6 fields per sample. ( D – F ) Immunohistochemistry staining and quantification of cleaved caspase-3 + CD11b + macrophages and MPO + neutrophils in the livers. MPO, myeloperoxidase. ( G ) The proportion of hepatic CD25 + Foxp3 + Tregs gated on CD3 + CD4 + T cells was analyzed by flow cytometry ( n = 9–10 samples/group). Scale bars, 100 μm ( A ); 20 μm ( C ); 50 μm ( D – F ). Data are presented as individual values and represent the mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001 versus nondiabetic controls by 1-way ANOVA.

Article Snippet: CD11b and active β-catenin double-positive macrophages were identified respectively by primary rabbit anti-mouse CD11b (AF6396, Beyotime) and rabbit monoclonal Ab active β-catenin (non-phospho-Ser33/37/Thr41) (8814, Cell Signaling Technology).

Techniques: Staining, TUNEL Assay, Immunohistochemistry, Flow Cytometry